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biotinylated secondary antibody  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated secondary antibody
    Biotinylated Secondary Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/SA-5488-1/DyLight+488+Streptavidin/pm41986348-458-17-24
    Average 95 stars, based on 192 article reviews
    biotinylated secondary antibody - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Changes in Spinal Neural Circuit Plasticity in a Rat Sciatic Nerve Transection Model
    Article Snippet: Next, the sections were incubated overnight at 4°C in 150 μL blocking solution containing anti‐rabbit vAchT (1:300; Synaptic Systems, Cat# 139103) and anti‐guinea pig vGlut1 (1:500; Millipore, Cat# AB5905). .. Then, the sections were incubated for 2 h at room temperature in 150 μL blocking solution containing goat‐anti‐rabbit Alexa Fluor Plus 594 (1:300; Invitrogen, Cat# A32740), goat‐anti‐guinea pig Alexa Fluor 647 (1:300; Invitrogen, Cat# A‐21450), and Streptavidin‐Dylight488 (1:150; Vector Laboratories, Cat# SA‐5488) for BDA labeling. .. Finally, the sections were cleared for 1 h using Rapi‐Clear 1.47 (SunJin Lab, Cat# RC147001), mounted on slide glasses, and covered with a cover slip using Rapi‐Clear 1.47 as the mounting medium.

    Article Title: Changes in Spinal Neural Circuit Plasticity in a Rat Sciatic Nerve Transection Model.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative sections were incubated overnight at 4◦C in 150 μL blocking solution containing anti-rabbit vAchT (1:300; Synaptic Systems, Cat# 139103) and anti-guinea pig vGlut1 (1:500; Millipore, Cat# AB5905). .. Then, the sections were incubated for 2 h at room temperature in 150 μL blocking solution containing goat-antirabbit Alexa Fluor Plus 594 (1:300; Invitrogen, Cat# A32740), goat-anti-guinea pig Alexa Fluor 647 (1:300; Invitrogen, Cat# A21450), and Streptavidin-Dylight488 (1:150; Vector Laboratories, Cat# SA-5488) for BDA labeling. .. Finally, the sections were cleared for 1 h using Rapi-Clear 1.47 (SunJin Lab, Cat# RC147001), mounted on slide glasses, and covered with a cover slip using Rapi-Clear 1.47 as the mounting medium.

    Article Title: Cytotoxic activity of pyrimethanil on human testicular teratocarcinoma NT2/D1 cells
    Article Snippet: Upon overnight incubation, appropriate secondary antibodies, biotinylated goat anti-rabbit IgG (BA-1000) or horse anti-mouse IgG (BA-2000) (Vector Laboratories, Burlingame, CA, U. S.) diluted 1:250 in 1 % BSA and PBS and 0.1 % Triton X-100 were applied for 1 h at RT. .. For fluorescence detection, cells were incubated for 1 h at RT in DyLight 488 Streptavidin (SA-5488) (Vector Laboratories, Burlingame, CA, U. S.) or DyLight 594 Streptavidin (SA-5594) (Vector Laboratories, Burlingame, CA, U. S.) diluted 1:500 in PBS. .. Staining of actin filaments (F-actin) was achieved by incubating cells for 1 h at RT in CytoPainter Phalloidin-iFluor 594 Reagent (ab176757, Abcam), diluted 1:3000 in PBS.

    Blocking Assay:

    Article Title: Changes in Spinal Neural Circuit Plasticity in a Rat Sciatic Nerve Transection Model
    Article Snippet: Next, the sections were incubated overnight at 4°C in 150 μL blocking solution containing anti‐rabbit vAchT (1:300; Synaptic Systems, Cat# 139103) and anti‐guinea pig vGlut1 (1:500; Millipore, Cat# AB5905). .. Then, the sections were incubated for 2 h at room temperature in 150 μL blocking solution containing goat‐anti‐rabbit Alexa Fluor Plus 594 (1:300; Invitrogen, Cat# A32740), goat‐anti‐guinea pig Alexa Fluor 647 (1:300; Invitrogen, Cat# A‐21450), and Streptavidin‐Dylight488 (1:150; Vector Laboratories, Cat# SA‐5488) for BDA labeling. .. Finally, the sections were cleared for 1 h using Rapi‐Clear 1.47 (SunJin Lab, Cat# RC147001), mounted on slide glasses, and covered with a cover slip using Rapi‐Clear 1.47 as the mounting medium.

    Article Title: Changes in Spinal Neural Circuit Plasticity in a Rat Sciatic Nerve Transection Model.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative sections were incubated overnight at 4◦C in 150 μL blocking solution containing anti-rabbit vAchT (1:300; Synaptic Systems, Cat# 139103) and anti-guinea pig vGlut1 (1:500; Millipore, Cat# AB5905). .. Then, the sections were incubated for 2 h at room temperature in 150 μL blocking solution containing goat-antirabbit Alexa Fluor Plus 594 (1:300; Invitrogen, Cat# A32740), goat-anti-guinea pig Alexa Fluor 647 (1:300; Invitrogen, Cat# A21450), and Streptavidin-Dylight488 (1:150; Vector Laboratories, Cat# SA-5488) for BDA labeling. .. Finally, the sections were cleared for 1 h using Rapi-Clear 1.47 (SunJin Lab, Cat# RC147001), mounted on slide glasses, and covered with a cover slip using Rapi-Clear 1.47 as the mounting medium.

    Labeling:

    Article Title: Changes in Spinal Neural Circuit Plasticity in a Rat Sciatic Nerve Transection Model
    Article Snippet: Next, the sections were incubated overnight at 4°C in 150 μL blocking solution containing anti‐rabbit vAchT (1:300; Synaptic Systems, Cat# 139103) and anti‐guinea pig vGlut1 (1:500; Millipore, Cat# AB5905). .. Then, the sections were incubated for 2 h at room temperature in 150 μL blocking solution containing goat‐anti‐rabbit Alexa Fluor Plus 594 (1:300; Invitrogen, Cat# A32740), goat‐anti‐guinea pig Alexa Fluor 647 (1:300; Invitrogen, Cat# A‐21450), and Streptavidin‐Dylight488 (1:150; Vector Laboratories, Cat# SA‐5488) for BDA labeling. .. Finally, the sections were cleared for 1 h using Rapi‐Clear 1.47 (SunJin Lab, Cat# RC147001), mounted on slide glasses, and covered with a cover slip using Rapi‐Clear 1.47 as the mounting medium.

    Article Title: Changes in Spinal Neural Circuit Plasticity in a Rat Sciatic Nerve Transection Model.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative sections were incubated overnight at 4◦C in 150 μL blocking solution containing anti-rabbit vAchT (1:300; Synaptic Systems, Cat# 139103) and anti-guinea pig vGlut1 (1:500; Millipore, Cat# AB5905). .. Then, the sections were incubated for 2 h at room temperature in 150 μL blocking solution containing goat-antirabbit Alexa Fluor Plus 594 (1:300; Invitrogen, Cat# A32740), goat-anti-guinea pig Alexa Fluor 647 (1:300; Invitrogen, Cat# A21450), and Streptavidin-Dylight488 (1:150; Vector Laboratories, Cat# SA-5488) for BDA labeling. .. Finally, the sections were cleared for 1 h using Rapi-Clear 1.47 (SunJin Lab, Cat# RC147001), mounted on slide glasses, and covered with a cover slip using Rapi-Clear 1.47 as the mounting medium.

    Staining:

    Article Title: Spliceosome induction is a druggable dependency of RAS-driven senescence and cancer.
    Article Snippet: .. For lung sections, YFP was stained with a GFP antibody followed by amplification of signal using a biotinylated secondary antibody and DyLight® 488 Streptavidin (Vector Laboratories). .. Afterwards, sections were incubated with 1μg/ml DAPI (Sigma) for 10min, washed with PBS and ddH2O, and mounted with Fluoromount-G aqueous mounting solution (SouthernBiotech, 0100-01).

    Amplification:

    Article Title: Spliceosome induction is a druggable dependency of RAS-driven senescence and cancer.
    Article Snippet: .. For lung sections, YFP was stained with a GFP antibody followed by amplification of signal using a biotinylated secondary antibody and DyLight® 488 Streptavidin (Vector Laboratories). .. Afterwards, sections were incubated with 1μg/ml DAPI (Sigma) for 10min, washed with PBS and ddH2O, and mounted with Fluoromount-G aqueous mounting solution (SouthernBiotech, 0100-01).

    Fluorescence:

    Article Title: Cytotoxic activity of pyrimethanil on human testicular teratocarcinoma NT2/D1 cells
    Article Snippet: Upon overnight incubation, appropriate secondary antibodies, biotinylated goat anti-rabbit IgG (BA-1000) or horse anti-mouse IgG (BA-2000) (Vector Laboratories, Burlingame, CA, U. S.) diluted 1:250 in 1 % BSA and PBS and 0.1 % Triton X-100 were applied for 1 h at RT. .. For fluorescence detection, cells were incubated for 1 h at RT in DyLight 488 Streptavidin (SA-5488) (Vector Laboratories, Burlingame, CA, U. S.) or DyLight 594 Streptavidin (SA-5594) (Vector Laboratories, Burlingame, CA, U. S.) diluted 1:500 in PBS. .. Staining of actin filaments (F-actin) was achieved by incubating cells for 1 h at RT in CytoPainter Phalloidin-iFluor 594 Reagent (ab176757, Abcam), diluted 1:3000 in PBS.

    other:

    Article Title: Intracellular interaction between FcRY receptor and IgY in ovarian vascular endothelial cells during avian maternal IgY transfer
    Article Snippet: The cells were then washed and incubated with Dylight488-conjugated streptavidin (1:100 in PBS; SA-5488, Vector Laboratories) for 30 min in a dark room.



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